Intermediate

Part:BBa_K390029

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)


GFP with 5'UTR and consensus RBS from Synechocystis sp. PCC 6803

This part is the cycle 3 mutant GFP (BBa_K208000) with the Synechocystis 5'UTR and consensus RBS (BBa_K390002).

It will be preceded by a series of promoters to assess their expression levels and conditions of activation. It will also be used to determine if expression levels on those promoters differs depending on the sequence of the RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 578
    Illegal XhoI site found at 479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None